# User:Melissa Novy/Notebook/CHEM-572/2013/02/20

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## Objectives

• Calculate the amount of AgNO3 to add to LB media solutions for desired final [Ag+].
• Incubate the silver-doped LB media solutions for 15 hours to monitor bacterial growth.
• Remove PLA films, made on 2013/02/19, from glass molds.
• Soak PLA films in pure H2O to test for Ag+ leaching with a silver ion selective electrode.

## Calculations

• Two sample calculations are given as examples. The final [Ag+] in 50-mL solutions of LB media was calculated as follows:
```  1 μM Ag+ in 50 mL of LB media
(0.0105 M Ag+) × V1 = (1×10-6 M) × (50 mL)
V1 = 0.00476 mL of 0.0105 M AgNO3
```
```  10 μM Ag+ in 50 mL of LB media
(0.1 M Ag+) × V1 = (10×10-6 M) × (50 mL)
V1 = 0.005 mL of 0.1 M AgNO3
```
• AgNO3 solutions made on 2013/02/05 and 2013/02/13 were added to the LB media solutions in the following amounts:
 Final [Ag+] Volume of AgNO3 Solution Added 1 μM 4.76 μL of 0.0105 M 10 μM 5.00 μL of 0.1 M 100 μM 50.0 μL of 0.1 M 1 mM 500 μL of 0.1 M 10 mM 5000 μL of 0.1 M
• Note that for each volume of AgNO3 added to the LB media, the same volume of LB media was first removed from the flask to maintain the final concentration of Ag+.