- Standardize 1 M NaHCO3 solution.
- Soak PVOH (MW 22000) in HPLC grade H2O to determine change in mass due to swelling.
- Soak PVOH (MW 22000), 1 mL glutaraldehyde, 1 mL porphyrin in 2 wt% H2SO4 until the film turns green. Then soak the film in salt solution and take UV-vis of the film.
PVOH (MW 22000) Film Swelling
- Change in mass of PVOH (MW 22000) films after soaking in 10 mL HPLC grade H2O for three days.
||Initial Mass [g]
||Final Mass [g]
||% Change 
| PVOH(22k),1mLGA,1mLPP,less colored||10/24/2012||0.014||0.0241||0.0101||72.14285714
- The films that displayed the smallest percent change in mass were PVOH(22K)+0.8wt%GA+100%CECMT (exchanged with CuSO4) and PVOH(22K)+1mLGA+1mLPP.
- The films that displayed the largest percent change in mass were PVOH(22K)+0.5mLGA+100%CECMT, PVOH(22K)+0.5mLGA+NaMT, and PVOH(22K)+0.5mLGA+1mLPP.
- Effect of ion exchange?
- It appears that adding 0.5 mL of glutaraldehyde to the films does not induce sufficient crosslinking to prevent swelling. However, 1 mL of glutaraldehyde seemed to reduce the degree to which the films swelled.
- Overall, most films displayed about a 100% increase in mass. In other words, their mass approximately doubled due to swelling.
Standardization of NaHCO3
- Weigh out 20 g of potassium hydrogen phthalate (KHP).
- The mass was divided into thirds, and then dried in the oven at 90°C for 48 hours.
- Each sample was divided in half again to give six samples of KHP total.
- One sample was placed in a 100 mL beaker and 2 drops of bromothymol blue was added.
- 1 M NaHCO3 was poured into a titration buret and the initial volume was recorded.
- The KHP was titrated with the NaHCO3 solution until the solution in the beaker turned blue and stopped fizzing.
- The process was repeated for two more KHP samples.
- The number of moles in the NaHCO3 solution was calculated.