User:Melvin Colorado Escobar/Notebook/581/2014/10/24: Difference between revisions
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==Objective== | ==Objective== | ||
Make a column for column chromatography. | |||
==Description== | ==Description== | ||
# | # Column Packing | ||
## The column was made with a syringe with a stop cock at the bottom. | |||
## We packed the column with glass wool and washed it with methanol to help keep it packed. | |||
## A layer of sand made of rocks from outside (mortar and pestle) is added next | |||
## A solution of 9.9999g of Silica in methanol was poured into the column. | |||
##* One must wait until the column settles. | |||
## The column was washed with deionized water until the column ran clear. | |||
## 10mL of BPA and let it run into the layer of silica. | |||
## 10mL of water was run through the column, and then 30mL of methanol were run into the column | |||
##* These were divided into 8 5mL fractions. | |||
[[Category:Course]] | [[Category:Course]] |
Revision as of 15:09, 28 November 2014
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ObjectiveMake a column for column chromatography. Description
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