User:Meng Xiao He/Notebook/fall08/2008/10/24: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
==PCR flanking regions==
* Insert content here...
Phusion:
 
* cbb3III 5
* cbb3I 3
* ccoII 5
* ccoIII 3
* DuoII 5
* DuoI 3


35 cycles:
*60°C annealing for 5 cycles, 58°C annealing for 30 cycles, 60°C for 5 cycles
*1kb extension time
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Revision as of 13:11, 24 October 2008

<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

PCR flanking regions

Phusion:

  • cbb3III 5
  • cbb3I 3
  • ccoII 5
  • ccoIII 3
  • DuoII 5
  • DuoI 3

35 cycles:

  • 60°C annealing for 5 cycles, 58°C annealing for 30 cycles, 60°C for 5 cycles
  • 1kb extension time


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