User:Meng Xiao He/Notebook/fall08/2008/10/24: Difference between revisions
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Mix: | |||
*2μL 25μM primer pair mix | |||
*25μL 2x master mix | |||
*colony | |||
*23μL H<sub>2</sub>O | |||
__NOTOC__ | __NOTOC__ |
Revision as of 13:13, 24 October 2008
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PCR flanking regionsPhusion:
35 cycles:
|
Mix:
- 2μL 25μM primer pair mix
- 25μL 2x master mix
- colony
- 23μL H2O