User:Meng Xiao He/Notebook/fall08/2008/11/01: Difference between revisions
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Since X over products were not very concentrated and had other amplicons, set up 25ul PCRs of just those 3 using some purified product as the template. | Since X over products were not very concentrated and had other amplicons, set up 25ul PCRs of just those 3 using some purified product as the template. | ||
PCR conditions: MXFG program, column 5, extension time changed to 45s. | PCR conditions: MXFG program, column 5, extension time changed to 45s. | ||
* This PCR ended up being very messy, with lots of smearing. There also appeared to be amplication of the fragments that made up the x-over individually. ?? | |||
==XmaI o/n digest== | |||
*cbb, cco, Duo from Xover PCR (original) [45, 70, 60 ul of DNA digested+ 2 of enzyme and appropriate vol of NEB4] | |||
*pUC19 | |||
*pER21 old, concentrated old, and new | |||
**vectors were 20ul DNA+1 of enzyme and necessary NEB4 | |||
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Revision as of 21:10, 1 November 2008
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PCR results
Lanes
X over product PCRSince X over products were not very concentrated and had other amplicons, set up 25ul PCRs of just those 3 using some purified product as the template. PCR conditions: MXFG program, column 5, extension time changed to 45s.
XmaI o/n digest
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