User:Meng Xiao He/Notebook/fall08/2008/11/03: Difference between revisions
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==Ligations== | ==Ligations/transformations== | ||
*XmaI heat killed | *XmaI heat killed | ||
*vectors treated with ant. phosphatase | *vectors treated with ant. phosphatase | ||
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*used NEB T4 ligase b/c quick ligases have PEG | *used NEB T4 ligase b/c quick ligases have PEG | ||
'DH5a': | |||
*TOPO + each flank's original PCR | |||
'TOP10': | |||
*pUC19 + each flank | |||
'pir+ electrocomp cells': | |||
*each pER21 prep + each flank | |||
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Revision as of 21:14, 1 November 2008
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Ligations/transformations
'DH5a':
'TOP10':
'pir+ electrocomp cells':
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