User:Meng Xiao He/Notebook/fall08/2008/11/03: Difference between revisions
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'''DH5a''', Kan+Xgal: | '''DH5a''', Kan+Xgal: | ||
*TOPO + each flank's original PCR | *TOPO + each flank's original PCR | ||
*pUC19 + each flank | *pUC19 + each flank | ||
*pUC19 neg control | |||
*4ul per transformation | |||
'''pir+ electrocomp cells''', LB spread w/ Gm+Xgal: | '''pir+ electrocomp cells''', LB spread w/ Gm+Xgal: | ||
*each pER21 prep + each flank | *each pER21 prep + each flank | ||
*pER21 neg control | |||
===Rx=== | ===Rx=== | ||
*8ul DNA mix, 1ul ligase, 1ul buffer | *8ul DNA mix, 1ul ligase, 1ul buffer | ||
*20 min ligation | *20 min ligation | ||
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Revision as of 08:26, 2 November 2008
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Ligations/transformations
DH5a, Kan+Xgal:
Rx
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