User:Meng Xiao He/Notebook/fall08/2008/11/05: Difference between revisions
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** 2ul pUC19 control from Invitrogen gave hundreds of colonies | ** 2ul pUC19 control from Invitrogen gave hundreds of colonies | ||
** Ligations w/ pER21 and flanks gave a few colonies each (<15)- 3 picked from each plate into liquid culture | ** Ligations w/ pER21 and flanks gave a few colonies each (<15)- 3 picked from each plate into liquid culture | ||
==Distinguishing digests== | |||
* TOPO (3.5kb): EcoRI+PstI | |||
** Duo: 1.2 kb, 300bp | |||
** cco: 1.7 kb | |||
** cbb3: 400, 830, 300 | |||
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Revision as of 20:52, 5 November 2008
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Transformation results
Distinguishing digests
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