User:Meng Xiao He/Notebook/fall08/2008/11/19: Difference between revisions
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==One primer PCR of suicide vectors== | ==One primer PCR of suicide vectors== | ||
* | * extension time: 4:30 | ||
* annealing temp: 52C | |||
* primer: 1ul (in 25ul of rx) of M13F primer from TOPO ki | |||
* cycles: 50 | |||
==Confirmation of pure cultures== | ==Confirmation of pure cultures== | ||
* PCR new TOPOA, pER-cco, pER-cbb | * PCR new TOPOA, pER-cco, pER-cbb |
Revision as of 16:08, 19 November 2008
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One primer PCR of suicide vectors
Confirmation of pure cultures
Sequencing
Loss of plasmid
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