User:Meng Xiao He/Notebook/fall08/2008/11/19: Difference between revisions
From OpenWetWare
Meng Xiao He (talk | contribs) |
Meng Xiao He (talk | contribs) |
||
Line 12: | Line 12: | ||
* primer: 1ul (in 25ul of rx) of M13F primer from TOPO ki | * primer: 1ul (in 25ul of rx) of M13F primer from TOPO ki | ||
* cycles: 50 | * cycles: 50 | ||
* DNA (old minipreps): 2ul each | |||
==Confirmation of pure cultures== | ==Confirmation of pure cultures== |
Revision as of 16:09, 19 November 2008
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |
One primer Phusion PCR of suicide vectors
Confirmation of pure cultures
Sequencing
Loss of plasmid
|