User:Meng Xiao He/Notebook/fall08/2008/11/19: Difference between revisions
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==Confirmation of pure cultures== | ==Confirmation of pure cultures== | ||
* PCR new | * Phusion PCR new TOPO cco, pER-cco, pER-cbb, pER-Duo#5, 7 colonies of TOPO C plate | ||
* Rx: 5ul mastermix, 4.3ul water, 0.5ul primer pair, touch of DNA/cells | |||
* Conditions: 2min initial denaturation at 98, 1 min extension, annealing at 57C, 35 cycles | |||
==Sequencing== | ==Sequencing== | ||
* pER-Duo | * pER-Duo |
Revision as of 16:33, 19 November 2008
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One primer Phusion PCR of suicide vectors
Confirmation of pure cultures
Sequencing
Loss of plasmid
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