User:Meng Xiao He/Notebook/fall08/2008/11/22: Difference between revisions
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** ones plated after (night of selection free growth → 4hrs of selection w/ Gm) | ** ones plated after (night of selection free growth → 4hrs of selection w/ Gm) | ||
==WM3064 transformants== | ==WM3064 transformants of KO vectors== | ||
* All are indeed DAP- | * All are indeed DAP- | ||
* Need to sequence to ensure whole plasmids transformed | * Need to sequence to ensure whole plasmids transformed | ||
* Glycerol stocks made | * Glycerol stocks made | ||
==Conjugation attempt== | ==Conjugation attempt== | ||
Revision as of 17:52, 22 November 2008
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PCR of suicide vectors
MR-1 KO electroporation plates
WM3064 transformants of KO vectors
Conjugation attempt | |
