User:Meng Xiao He/Notebook/fall08/2008/11/25: Difference between revisions

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*Duo streaked: 2 colonies
*Duo streaked: 2 colonies
*plating cell mix: this seems like a back idea, as significant amounts of E. coli growth occurs in patches (not isolated colonies)
*plating cell mix: this seems like a back idea, as significant amounts of E. coli growth occurs in patches (not isolated colonies)
===Colony PCR===
[[Image:11.25.pcr.mx.jpg]]
No colony from conjugation gave bands, just smears as in first few lanes.


==Transformation update==
==Transformation update==

Revision as of 15:47, 25 November 2008

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Conjugation results

Filter

  • cbb3, plate: >100 colonies, although sizes vary greatly, with many large
  • cbb3, liquid: MR-1 growth (from pellet color)
  • cco, liquid: looks like E. coli is growing? Pellet is white
  • Duo, plate: 2 colonies, medium sized
  • cbb3 and Duo, filter into liquid: both cultures thick, streaked for isolation on LB Gm plates
  • cco: no growth in filter+liquid yet, plated cultures had many (~50) white, colonies, that look like E. coli

Spin down

  • cbb3 streaked: scattered colonies at beginning of streak
  • cbb3, liquid: MR-1 growth (from pellet color)
  • cco, liquid: white pellet > E. coli?
  • Duo streaked: 2 colonies
  • plating cell mix: this seems like a back idea, as significant amounts of E. coli growth occurs in patches (not isolated colonies)

Colony PCR

No colony from conjugation gave bands, just smears as in first few lanes.

Transformation update

  • Old transformations (last set that had no intervening nonselective growth period) developped yellow-orange contaminant colonies. Clearly not E. coli/MR-1 b/c growth was minimal overnight. Plates discarded.
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