User:Meng Xiao He/Notebook/fall08/2008/11/27: Difference between revisions

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*1kb ladder
*1kb ladder
*cco MR-1 conjugant colonies 1, 2
*cco MR-1 conjugant colonies 1, 2
*cco wm plasmid PCR w/ ccoII5 and M13F primer to determine orientation
*ignore last, wrong setup
 
===Duo gradient, WM miniprep Sma digests===
===Duo gradient, WM miniprep Sma digests===
[[Image:11-27_gel2.mx.jpg]]
[[Image:11-27_gel2.mx.jpg]]

Revision as of 13:59, 27 November 2008

<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

PCR results

  • Gradient cycling temperatures (left to right): 55.4, 56.8, 58.0, 58.9
  • 2:15 extensions
  • min at 94 for initial denaturation
  • Platinum

cbb gradient, cco

  • 1-4: cbb3 plasmid (wm)
  • 5-8: cbb3 MR-1 conjugant colonies 1-4
  • 1kb ladder
  • cco MR-1 conjugant colonies 1, 2
  • ignore last, wrong setup

Duo gradient, WM miniprep Sma digests

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