User:Meng Xiao He/Notebook/fall08/2008/12/04: Difference between revisions
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* repatching of colonies from old cco plate: 1 colony was Gm sensitive, all were sucrose resistant | * repatching of colonies from old cco plate: 1 colony was Gm sensitive, all were sucrose resistant | ||
* patching of colonies from new cco plate: all 20 were sucrose resistant and none were Gm sensitive, no obvious morphological diffs on plate, although liquid cultures had appeared to grow a little slow | * patching of colonies from new cco plate: all 20 were sucrose resistant and none were Gm sensitive, no obvious morphological diffs on plate, although liquid cultures had appeared to grow a little slow | ||
* set up new MR-1, wmDuo, and wdcco cultures for conjugation tomorrow | |||
==PCR redo== | ==PCR redo== | ||
* Previous PCR had high MW smears. Unclear if anything worked. Remainder of PCR mix run on 58C long cycle PCR to see if there will be high MW amplication corresponding to a complete, unrecombined out suicide vector. | * Previous PCR had high MW smears. Unclear if anything worked. Remainder of PCR mix run on 58C long cycle PCR to see if there will be high MW amplication corresponding to a complete, unrecombined out suicide vector. | ||
Revision as of 13:26, 4 December 2008
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new cco colonies
PCR redo
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