User:Meng Xiao He/Notebook/fall08/2008/12/04: Difference between revisions

From OpenWetWare
< User:Meng Xiao He‎ | Notebook‎ | fall08‎ | 2008‎ | 12
Jump to navigationJump to search
No edit summary
(Undo revision 267439 by Meng Xiao He (Talk))
Line 2: Line 2:
{|{{table}} width="800"
{|{{table}} width="800"
|-
|-
|style="background-color: #F2F2F2" align="left"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />[[User:Meng_Xiao_He/Notebook/fall08/2008/12/01| Previous entry]]<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="left"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br /><html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[User:Meng_Xiao_He/Notebook/fall08/2008/12/01| Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}


|-
|-

Revision as of 13:31, 4 December 2008

<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html> Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

new cco colonies

  • repatching of colonies from old cco plate: 1 colony was Gm sensitive, all were sucrose resistant
  • patching of colonies from new cco plate: all 20 were sucrose resistant and none were Gm sensitive, no obvious morphological diffs on plate, although liquid cultures had appeared to grow a little slow
  • set up new MR-1, wmDuo, and wdcco cultures for conjugation tomorrow

PCR redo

  • Previous PCR had high MW smears. Unclear if anything worked. Remainder of PCR mix run on 58C long cycle PCR to see if there will be high MW amplication corresponding to a complete, unrecombined out suicide vector.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Meng_Xiao_He/Notebook/fall08/2008/12/04&oldid=267440"