User:Meng Xiao He/Notebook/fall08/2008/12/06: Difference between revisions

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==Platinum PCRs==
Products will be sequenced if any


*redo of ones that gave 500/1kb bands from 11/30 cco KO's: colonies 1, 3, 8, 9, 10
*brown cco: 3 from supernatant and 1 from pellet
*brown cbb: 2 from supernatant and 1 from pellet
25ul reactions, 40 cycles:
*7 min initial 94C denaturation
*30 s denature
*30 s annealing 58→56.5 in first 15 cycles by dropping 0.1 decrease/cycle, 59→56.5 in last 25 cycles
*2 min elongation
*10 min final extension


==conjugation, continued==
==conjugation, continued==

Revision as of 15:11, 7 December 2008

<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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conjugation, continued

  • cells vortexed off filter and plated on LB Gm

brown supernatants

  • cco and cbb KO (in salt-free LB) cultures have turned brown
  • supernatants and pellets cultured
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