User:Meng Xiao He/Notebook/fall08/2008/12/06: Difference between revisions

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==conjugation, continued==
==conjugation, continued==
* cells vortexed off filter and plated on LB Gm
* cells vortexed off filter and plated on LB Gm

Revision as of 22:06, 7 December 2008

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conjugation, continued

  • cells vortexed off filter and plated on LB Gm
  • remaining cells grown in LB Gm

brown supernatants

  • cco and cbb KO (in salt-free LB) cultures have turned brown
  • supernatants and pellets cultured
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