User:Meng Xiao He/Notebook/fall08/2008/12/07: Difference between revisions
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==KO thoughts== | ==KO thoughts== | ||
* maybe should supplement with ferric citrate? | * maybe should supplement with ferric citrate? | ||
** new LB/no salts cultures for new cco, cbb#3, and Duo#18, supplemented with 50mM ferric citrate | |||
* can it/ need it be done under anaerobic conditions? | * can it/ need it be done under anaerobic conditions? | ||
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Revision as of 15:13, 7 December 2008
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Platinum PCRsProducts will be sequenced if any
25ul reactions, 40 cycles:
conjugation, round 2
KO thoughts
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