User:Meng Xiao He/Notebook/fall08/2008/12/08: Difference between revisions
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==PCRs to determine proper insertion of suicide vector== | ==PCRs to determine proper insertion of suicide vector== | ||
Phusion used | Phusion used | ||
*Colonies: | |||
*WT, 1 of the old ones ("new cco", cbb3 #3, Duo #18), and 6 new ones | |||
**cco doesn't have 6 new ones, so use 1 brown supernatant and 1 brown streak colony | |||
*For each colony: 2 primer sets [flank outer+ 3R] and [flank outer+5F] | *For each colony: 2 primer sets [flank outer+ 3R] and [flank outer+5F] | ||
*Cycling conditions: | *Cycling conditions: |
Revision as of 16:06, 8 December 2008
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PCRs to determine proper insertion of suicide vectorPhusion used
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