User:Meng Xiao He/Notebook/fall08/2008/12/08: Difference between revisions
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#30s @ 59->56 in 15 cycles (drop 0.2 degrees per cycle) | #30s @ 59->56 in 15 cycles (drop 0.2 degrees per cycle) | ||
#30s @ 57 for 10 cycles | #30s @ 57 for 10 cycles | ||
#3:30 @ 72 (should capture WT cells as well) | #3:30 @ 72 (should capture WT cells as well). 45s for last 10 | ||
#5 min@ 72 to end | #5 min@ 72 to end | ||
#hold at 4 | #hold at 4 |
Revision as of 17:33, 8 December 2008
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PCRs to determine proper insertion of suicide vectorPhusion used
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