User:Meng Xiao He/Notebook/fall08/2008/12/08: Difference between revisions
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**cco doesn't have 6 new ones, so used 1 and 2 from 11/30 sucrose selection | **cco doesn't have 6 new ones, so used 1 and 2 from 11/30 sucrose selection | ||
*For each colony: 2 primer sets [flank outer+ 3R] and [flank outer+5F] (note all 3 flank outer sets were included- should be OK? sizes are 4.5, 5.5, 6.5 kb) | *For each colony: 2 primer sets [flank outer+ 3R] and [flank outer+5F] (note all 3 flank outer sets were included- should be OK? sizes are 4.5, 5.5, 6.5 kb) | ||
*Cycling conditions: | *Cycling conditions (program is '''12-8'''): | ||
#2min at 98 | #2min at 98 | ||
#7s at 98 | #7s at 98 |
Revision as of 15:52, 9 December 2008
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conjugation filter colonies
PCRs to determine proper insertion of suicide vectorPhusion used
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