User:Meng Xiao He/Notebook/fall08/2008/12/09: Difference between revisions

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===Samples with bands rerun at higher res==
===Samples with bands rerun at higher res===
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Revision as of 12:55, 9 December 2008

<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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12/7 Platinum PCR results

1KB ladder

  • unfortunately, EtBr seems to have run so far up gel that bands < 1kb can't be distinguished
  • 1-5 were colonies 1, 3, 8, 9, and 10 from the 11-30 "new cco" KO
  • then ladder
  • next 3: cbb from brown cultures
  • next 4: cco from brown cultures
    • colonies 1, 2, and possibly 4 have at least taken up the KO plasmid, even if it hasn't been removed from the genome, since the cco flank is itself 1.5 kb
    • these conditions work for platinum colony PCR for cco, at least

12/8 Phusion PCR results

Within each block (end with ladder): cco 3R > cco 5F > Duo 3R > Duo 5F > cbb 3R > cbb 5F

Blocks go: (new filter colony 1 > 2 > 3 > 4 > 5 > 6) > old colony > wt control

  • for Duo, old and new colony 6 flipped
  • for cco, new 5, 6 are actually 1, 2 from 11-30 Gm sensitive, sucrose R colonies ("new cco" KO)

Samples with bands rerun at higher res

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