User:Meng Xiao He/Notebook/fall08/2008/12/09: Difference between revisions
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* cbb colonies of interest: new 2, new 3, new4, new 5, new 6 | * cbb colonies of interest: new 2, new 3, new4, new 5, new 6 | ||
* cco colonies of interest: new 1, new 2 | * cco colonies of interest: new 1, new 2 | ||
== | ==Insertional colony processing== | ||
* restreaked on LB Gm plates | |||
===LB minus salt:=== | |||
* Duo new # 1 (rename to Duo12.5A) | * Duo new # 1 (rename to Duo12.5A) | ||
* cco new # 3 (rename to cco12.5A) | * cco new # 3 (rename to cco12.5A) | ||
===supplemented with 20ul ferric citrate=== | ====supplemented with 20ul ferric citrate==== | ||
* Duo brown # 1 (rename to Duo12.5B) | * Duo brown # 1 (rename to Duo12.5B) | ||
* cco new # 5 (rename to cco12.5B) | * cco new # 5 (rename to cco12.5B) |
Revision as of 13:18, 9 December 2008
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |
12/7 Platinum PCR results1KB ladder
12/8 Phusion PCR resultsWithin each block (end with ladder): cco 3R > cco 5F > Duo 3R > Duo 5F > cbb 3R > cbb 5F Blocks go: (new filter colony 1 > 2 > 3 > 4 > 5 > 6) > old colony > wt control
Samples with bands rerun at higher res
Conclusions
Insertional colony processing
LB minus salt:
supplemented with 20ul ferric citrate
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