User:Meng Xiao He/Notebook/fall08/2008/12/09: Difference between revisions
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====Conclusions==== | ====Conclusions==== | ||
* program used ('''12/8''' on big room machine) seems to work (at least some of the time) with the outer primer sets (in this case, all 3 were added) | * program used ('''12/8''' on big room machine) seems to work (at least some of the time) with the outer primer sets (in this case, all 3 were added) | ||
* cbb colonies of interest: new 2, new 3 | * cbb colonies of interest: [(new 2, new 3)→ambiguous b/c cannot see if the WT 5.4kb cbb outer fragment is indeed missing] | ||
**new4, new 5, new 6 don't look like they incorporated the vector, since the WT 5.4kb cbb outer fragment is still there- what are those bands btw 1.6kb and 2kb? | |||
* cco colonies of interest: new 1, new 2 | * cco colonies of interest: new 1, new 2 | ||
Revision as of 10:47, 11 December 2008
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12/7 Platinum PCR results1KB ladder
12/8 Phusion PCR resultsWithin each block (end with ladder): cco 3R > cco 5F > Duo 3R > Duo 5F > cbb 3R > cbb 5F Blocks go: (new filter colony 1 > 2 > 3 > 4 > 5 > 6) > old colony > wt control
Remainder of samples with bands rerun at higher res
Conclusions
Insertional colony processing
LB minus salt:
supplemented with 20ul ferric citrate
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