User:Meng Xiao He/Notebook/fall08/2008/12/10: Difference between revisions
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==PCR tests== | ==PCR tests== | ||
*use 12-8 protocol on | *use 12-8 protocol on cco12.9B, C: each takes cco5F, cco3R, and just pair of 3 outers | ||
*use Platinum adaptation of 12-8 protocol: | *use Platinum adaptation of 12-8 protocol: | ||
:Cycling conditions: | :Cycling conditions: | ||
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#10 min@ 72 to end | #10 min@ 72 to end | ||
#hold at 4 | #hold at 4 | ||
**61: cbb12.9A, B, C, cco12.9D; 57: cbb12.9D, E, cco12.9A | |||
==Plating on sucrose== | ==Plating on sucrose== |
Revision as of 15:41, 10 December 2008
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PCR tests
Plating on sucrose
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