User:Meng Xiao He/Notebook/fall08/2008/12/10: Difference between revisions
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==PCR tests== | ==PCR tests== | ||
*use 12-8 protocol on cco12.9B, C: each takes cco5F, cco3R, and just pair of 3 outers | *use 12-8 protocol on cco12.9B, C: each takes cco5F, cco3R, and just pair of 3 outers, 20ul | ||
*use Platinum adaptation of 12-8 protocol: | *use Platinum adaptation of 12-8 protocol: 25ul | ||
:Cycling conditions: | :Cycling conditions: | ||
#5 min at 94 | #5 min at 94 |
Revision as of 15:42, 10 December 2008
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PCR tests
Plating on sucrose
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