User:Meng Xiao He/Notebook/fall08/2008/12/10: Difference between revisions
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==PCR tests== | ==PCR tests== | ||
*use 12-8 protocol on | *use 12-8 protocol on cco12.9B, C: each takes cco5F, cco3R, and just pair of 3 outers, 20ul | ||
*use Platinum adaptation of 12-8 protocol: | *('''update: does not work''') use Platinum adaptation of 12-8 protocol: 25ul | ||
:Cycling conditions: | :Cycling conditions: | ||
#5 min at 94 | #5 min at 94 | ||
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#10 min@ 72 to end | #10 min@ 72 to end | ||
#hold at 4 | #hold at 4 | ||
::61: cbb12.9A, B, C, cco12.9D; 57: cbb12.9D, E, cco12.9A | |||
==Plating on sucrose== | ==Plating== | ||
*1: | ===on sucrose=== | ||
* | *1:2 dilution into fresh salt-free-LB, let grow 1hr, then plate 10ul | ||
** cultures were very thin (may be because had to user rocker- shaker is nonfunctional) | |||
** cco12.9A grew thick, though | |||
===on Gm=== | |||
*remaining Gm culture of wash off from filter of Duo | |||
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Latest revision as of 10:17, 12 December 2008
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PCR tests
Platingon sucrose
on Gm
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