User:Meng Xiao He/Notebook/fall08/2008/12/11: Difference between revisions
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==PCR== | |||
[[Image:12-11pcr1.jpg]] | |||
lanes 1-6 (Phusion, 12-8 program): | |||
*cco12.9B outers+cco5F, outers+cco3R, outers+water | |||
*cco12.9C outers+cco5F, outers+cco3R, outers+water | |||
* 1kb, 100 bp ladders | |||
* Platinum (annealing temp of 61) cco12.9D outers+cco5F, outers+cco3R, outers+water | |||
==Replating== | ==Replating== | ||
* Duo on Gm looked very messy (lots of tiny colonies in first part of streak, along with slightly bigger ones, can't tell if is mix of MR-1 and E. coli) > restreaked and let grow | * Duo on Gm looked very messy (lots of tiny colonies in first part of streak, along with slightly bigger ones, can't tell if is mix of MR-1 and E. coli) > restreaked and let grow | ||
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Revision as of 10:31, 11 December 2008
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PCR
Replating
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