User:Meng Xiao He/Notebook/fall08/2008/12/11: Difference between revisions
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==PCR== | ==PCR== | ||
[[Image:12-11pcr1.jpg]] | [[Image:12-11pcr1.jpg]] | ||
lanes 1-6 (Phusion, 12-8 program): | lanes 1-6 (Phusion, 12-8 program): | ||
*cco12.9B outers+cco5F, outers+cco3R, outers+water | *cco12.9B outers+cco5F, outers+cco3R, outers+water | ||
** this particular cell is WT, but does not show the band seen [[User:Meng_Xiao_He/Notebook/fall08/2008/12/09#12.2F7_Platinum_PCR_results| here]]? | |||
*cco12.9C outers+cco5F, outers+cco3R, outers+water | *cco12.9C outers+cco5F, outers+cco3R, outers+water | ||
**Confused... why are there 2 bands? Unless it's actually a KO??? | |||
* 1kb, 100 bp ladders | * 1kb, 100 bp ladders | ||
* Platinum (annealing temp of 61) cco12.9D outers+cco5F, outers+cco3R, outers+water | * Platinum (annealing temp of 61) cco12.9D outers+cco5F, outers+cco3R, outers+water | ||
Platinum protocols seems not to work... | |||
==Replating== | ==Replating== | ||
* Duo on Gm looked very messy (lots of tiny colonies in first part of streak, along with slightly bigger ones, can't tell if is mix of MR-1 and E. coli) > restreaked and let grow | * Duo on Gm looked very messy (lots of tiny colonies in first part of streak, along with slightly bigger ones, can't tell if is mix of MR-1 and E. coli) > restreaked and let grow | ||
* all the sucrose plates were lawns, additionally, concentrated ferric citrate seems to be a bad thing (middle of plate, where ferric citrate was added was clear for both B colonies) | * all the sucrose plates were lawns, additionally, concentrated ferric citrate seems to be a bad thing (middle of plate, where ferric citrate was added was clear for both B colonies) | ||
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Latest revision as of 10:18, 12 December 2008
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PCR
lanes 1-6 (Phusion, 12-8 program):
Platinum protocols seems not to work... Replating
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