User:Meng Xiao He/Notebook/fall08/2008/12/11

From OpenWetWare
Jump to navigationJump to search
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html> Constants
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

PCR

lanes 1-6 (Phusion, 12-8 program):

  • cco12.9B outers+cco5F, outers+cco3R, outers+water
  • cco12.9C outers+cco5F, outers+cco3R, outers+water
  • 1kb, 100 bp ladders
  • Platinum (annealing temp of 61) cco12.9D outers+cco5F, outers+cco3R, outers+water

Replating

  • Duo on Gm looked very messy (lots of tiny colonies in first part of streak, along with slightly bigger ones, can't tell if is mix of MR-1 and E. coli) > restreaked and let grow
  • all the sucrose plates were lawns, additionally, concentrated ferric citrate seems to be a bad thing (middle of plate, where ferric citrate was added was clear for both B colonies)
    • restreaked, ferric citrate maintained for the B colonies


Bold text

Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Meng_Xiao_He/Notebook/fall08/2008/12/11&oldid=269367"