User:Meng Xiao He/Notebook/fall08/2008/12/12: Difference between revisions
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==Replating on Gm, sucrose== | ==Replating on Gm, sucrose== | ||
* colonies replated on both sucrose and Gm | * colonies replated on both sucrose and Gm | ||
* 5 cbb and 6 cco colonies Phusion PCRed using 12-8 | * 5 cbb and 6 cco colonies Phusion PCRed using PhFLANK program (12-8 modified to have 3:45 extension) > note that flanking pairs were only the ko gene and Duo as the control (no orientation primer) | ||
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Revision as of 10:29, 12 December 2008
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Replating on Gm, sucrose
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