User:Meng Xiao He/Notebook/fall08/2008/12/13: Difference between revisions
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[[Image:12-13pcr.jpg]] | [[Image:12-13pcr.jpg]] | ||
*PCR seems to have failed even more than usual | *PCR seems to have failed even more than usual | ||
** cco colonies 1 and 5 are definitely WT (either revertants or previous PCR just didn't amplify the band properly) | |||
*new PCRs set up with colonies that are sucroseR but not GmR | *new PCRs set up with colonies that are sucroseR but not GmR | ||
** 20ul reaction, revert back to 12-8 in case longer extension time is doing bad things... | ** 20ul reaction, revert back to 12-8 in case longer extension time is doing bad things... | ||
*** All of the PCRs failed except for mess at bottom... maybe try fewer cells and alter reaction volume? Will try to replicate 12-8 PCR conditions exactly | |||
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Latest revision as of 15:58, 13 December 2008
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page<html> </html> Constants <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |
First 6 are cco, next 5 are cbb
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