User:Meng Xiao He/Notebook/fall08/2008/12/13

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[[Image:12-13pcr.jpg]]
[[Image:12-13pcr.jpg]]
*PCR seems to have failed even more than usual
*PCR seems to have failed even more than usual
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** cco colonies 1 and 5 are definitely WT (either revertants or previous PCR just didn't amplify the band properly)
*new PCRs set up with colonies that are sucroseR but not GmR
*new PCRs set up with colonies that are sucroseR but not GmR
** 20ul reaction, revert back to 12-8 in case longer extension time is doing bad things...
** 20ul reaction, revert back to 12-8 in case longer extension time is doing bad things...
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Revision as of 12:04, 13 December 2008

Main project page       Constants
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First 6 are cco, next 5 are cbb

Image:12-13pcr.jpg

  • PCR seems to have failed even more than usual
    • cco colonies 1 and 5 are definitely WT (either revertants or previous PCR just didn't amplify the band properly)
  • new PCRs set up with colonies that are sucroseR but not GmR
    • 20ul reaction, revert back to 12-8 in case longer extension time is doing bad things...
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