User:Meng Xiao He/Notebook/fall08/2008/12/15

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(PCR retry)
Current revision (12:27, 16 December 2008) (view source)
(PCR retry)
 
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[[Image:2008-12-15_pcr_plate.jpg]]
[[Image:2008-12-15_pcr_plate.jpg]]
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However, it seems that the ones with bands are the only PCRs that remotely worked; the bands are actually the upper bands:
 +
[[Image:12-16pcr_redo.jpg]]
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Will have to troubleshoot this PCR...
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PCR retry

  • retried PCR using 15ul volume and all 3 outer primer sets, 12-8 conditions
  • note that 24 lane means sets of 12 are staggered:

Image:2008-12-15_pcr_plate.jpg

However, it seems that the ones with bands are the only PCRs that remotely worked; the bands are actually the upper bands: Image:12-16pcr_redo.jpg

Will have to troubleshoot this PCR...

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