User:Meng Xiao He/Notebook/fall08/2009/01/07: Difference between revisions

From OpenWetWare
< User:Meng Xiao He‎ | Notebook‎ | fall08‎ | 2009‎ | 01
Jump to navigationJump to search
Line 21: Line 21:
The lane order is the same as in the bullets, except for the first 3 lanes, which has cco#18 > cco#34 > cco#21
The lane order is the same as in the bullets, except for the first 3 lanes, which has cco#18 > cco#34 > cco#21
*lane 1 (cco#18) and lane 6 (cco#31) are the only candidates for loss> there does appear to be banding of some sort > Xover primer set nested PCR candidate
*lane 1 (cco#18) and lane 6 (cco#31) are the only candidates for loss> there does appear to be banding of some sort > Xover primer set nested PCR candidate
*lane 5 not explained: did not lose WT copy, and lower band not right size for flanks
<!-- end -->
<!-- end -->
|}
|}

Revision as of 14:34, 7 January 2009

<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html> Constants
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

PCR from yesterday

  • 1st block conditions: 12-8 program, unmodified
  • 2nd block conditions: [[[--- DMSO, seems to work much better
  • 3rd block conditions: Platinum

Rerun

The following from above were rerun:

  • lane 1: cco # 34
  • lanes 20-30: cco (18, 21, 30, 31, 32), cbb (25, 26, 27, 28, 29)

The lane order is the same as in the bullets, except for the first 3 lanes, which has cco#18 > cco#34 > cco#21

  • lane 1 (cco#18) and lane 6 (cco#31) are the only candidates for loss> there does appear to be banding of some sort > Xover primer set nested PCR candidate
  • lane 5 not explained: did not lose WT copy, and lower band not right size for flanks
Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Meng_Xiao_He/Notebook/fall08/2009/01/07&oldid=275447"