User:Meng Xiao He/Notebook/fall08/2009/01/07: Difference between revisions
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==PCR from yesterday== | ==PCR from yesterday== | ||
Phusion runs were with the outer primer set (only the specific pair, no others), Platinum runs were with X over primer set | |||
[[Image:2009-01-07_pcr.jpg]] | [[Image:2009-01-07_pcr.jpg]] | ||
*1st block conditions: Phusion, 12-8 program, unmodified | *1st block conditions: Phusion, 12-8 program, unmodified | ||
*2nd block conditions: 3% DMSO + Phusion, in gradient thermocycler under '''1-6DMSO''', seems to work much better | *2nd block conditions: 3% DMSO + Phusion, in gradient thermocycler under '''1-6DMSO''', seems to work much better | ||
*3rd block conditions: Platinum | *3rd block conditions: Platinum, with Xover primer set | ||
===Rerun=== | ===Rerun=== | ||
The following from above were rerun: | The following from above were rerun: | ||
Latest revision as of 10:36, 8 January 2009
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PCR from yesterdayPhusion runs were with the outer primer set (only the specific pair, no others), Platinum runs were with X over primer set
RerunThe following from above were rerun:
The lane order is the same as in the bullets, except for the first 3 lanes, which has cco#18 > cco#34 > cco#21
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