User:Meng Xiao He/Notebook/fall08/Constants: Difference between revisions
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=PCR= | =PCR= | ||
==Platinum== | ==Platinum== | ||
===cco MR-1 colony=== | |||
'''12-7''' on big room machine: | |||
25ul reactions, 40 cycles: | |||
*7 min initial 94C denaturation | |||
*30 s denature | |||
*30 s annealing 58→56.5 in first 15 cycles by dropping 0.1 decrease/cycle, 59→56.5 in last 25 cycles | |||
*2 min elongation | |||
*10 min final extension | |||
==Phusion== | ==Phusion== | ||
Revision as of 11:21, 9 December 2008
PCR
Platinum
cco MR-1 colony
12-7 on big room machine:
25ul reactions, 40 cycles:
- 7 min initial 94C denaturation
- 30 s denature
- 30 s annealing 58→56.5 in first 15 cycles by dropping 0.1 decrease/cycle, 59→56.5 in last 25 cycles
- 2 min elongation
- 10 min final extension
