|
|
| Line 6: |
Line 6: |
| | colspan="2"| | | | colspan="2"| |
| <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> |
| | | ==Objectives== |
| | | * Using UV/Vis, find which mole ratios of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] to [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] result in the most [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] unfolding and formation of gold nanoparticles. |
| ==Objective== | | * Make solutions with varying concentrations of Tris buffer and [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] fibers |
| *Make solutions of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] with varying mole ratios | |
| *Find optimal mole ratio of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] to [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] for formation of gold nanoparticles
| |
| ==Procedure== | | ==Procedure== |
| #A stock solution of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] was made, with the attempted Molarity of 10mM. | | #UV/Vis |
| ## (moles [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]])/.025L H<sub>2</sub>O = .01M | | ##1mL of each solution with a varying mole ratios of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] was inserted into a cuvette, which went into a UV/Vis Spectroscoper |
| ## 2.5*10<sup>-4</sup> mol [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]]
| | ##Spectra were obtained and peaks for gold nanoparticles were identified at the 520nm range. |
| ## 2.5*10<sup>-4</sup> mol * 339.785mol/g [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] = .0849g
| | ##Cuvette was cleaned between each spectra, and the same cuvette was used each time |
| ## .0197g of the .0849g stuck to the weigh paper, leaving .0656g[[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]].
| | #Tris buffer serial dilution |
| ## .0656g [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] * (1mol[[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]]/339.79g/mol[[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]])= xM [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]]
| | ##A .01M stock solution of Tris was made by putting .0121g Tris in 10mL H<sub>2</sub>O |
| ## .007721M [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]]
| | ### [(mass Tris)* 121.14g/mol Tris]/.01L = .01M Tris solution |
| ## .0849g [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] was put in 25mL to make a 10mM solution
| | ### .0121g Tris |
| #A 15μM stock solution of [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] was made
| | ## 1mL was taken from the tube with a pipette and moved to the next tube. 1mL from this tube was moved to the next tube, and so on until all get some amount of Tris. |
| ## (moles [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]])/.025L = .000015
| | ## 9mL H<sub>2</sub>O was put in the first tube to receive 1mL Tris from the stock solution. |
| ## 3.75*10^-7mol [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]]
| | ## A serial dilution was repeated with H<sub>2</sub>O rather than Tris. |
| ## 3.75*10^-7mol [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] * 66,463g/mol [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] = .0249g [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]]
| | ## Test tubes were wrapped and heated at at 80°C for 4 hours. |
| ## .0249g [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] was put in 25mL to make a 15μM solution
| |
| #Solutions were made with the mole ratios of ([[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]]/[[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]]) 60, 80, 100, 120, 128, 130, 132, 133, 134, 136, 138, 140, 160, and 170. Volume of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution was calculated for each tube and inserted. The water needed for each tube was calculated by subtracting the volume of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] stock solution from 6mL.
| |
| ##m<sub>1</sub>v<sub>1</sub>=m<sub>2</sub>v<sub>2</sub>
| |
| ##15μM*(volume [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] stock solution) = (1.5*6) | |
| ###0.6mL [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] stock solution in each tube | |
| ##7720μM*x mL=6mL*(1.5*60μM) | |
| ###0.069mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution | |
| ###5.331 mL H<sub>2</sub>O
| |
| ##7720μM*x mL=6mL*(1.5*80μM) | |
| ###0.093mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution | |
| ###5.307mL H<sub>2</sub>O
| |
| ##7720μM*x mL=6mL*(1.5*100μM)
| |
| ###0.117mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| |
| ###5.283mL H<sub>2</sub>O
| |
| ##7720μM*x mL=6mL*(1.5*120μM)
| |
| ###0.134mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| |
| ###5.266mL H<sub>2</sub>O
| |
| ##7720μM*x mL=6mL*(1.5*128μM)
| |
| ###0.149mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| |
| ###5.251mL H<sub>2</sub>O
| |
| ##7720μM*x mL=6mL*(1.5*130μM)
| |
| ###.152mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| |
| ###5.248mL H<sub>2</sub>O | |
| ##7720μM*x mL=6mL*(1.5*132μM) | |
| ###0.154mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| |
| ###5.246mL H<sub>2</sub>O | |
| ##7720μM*x mL=6mL*(1.5*133μM)
| |
| ###0.155mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| |
| ###5.245mL H<sub>2</sub>O
| |
| ##7720μM*x mL=6mL*(1.5*134μM)
| |
| ###0.156mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution | |
| ###5.254mL H<sub>2</sub>O
| |
| ##7720μM*x mL=6mL*(1.5*136μM)
| |
| ###0.159mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| |
| ###5.244mL H<sub>2</sub>O
| |
| ##7720μM*x mL=6mL*(1.5*138μM)
| |
| ###0.161mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| |
| ###5.242mL H<sub>2</sub>O
| |
| ##7720μM*x mL=6mL*(1.5*140μM)
| |
| ###0.163mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| |
| ###5.237mL H<sub>2</sub>O
| |
| ##7720μM*x mL=6mL*(1.5*160μM)
| |
| ###0.187mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| |
| ###5.213mL H<sub>2</sub>O
| |
| ##7720μM*x mL=6mL*(1.5*170μM)
| |
| ###0.198mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| |
| ###5.202mL H<sub>2</sub>O | |
| #The tubes were wrapped in tin foil and heated at 80°C for 4 hours.
| |
| <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
| |} | | |} |
|
| |
|
| __NOTOC__ | | __NOTOC__ |
Project name
