User:Michael F. Nagle/Notebook/Chem 571/2012/08/29: Difference between revisions

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==Objective==
==Objective==
*Make stock solutions of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]]
*Make stock solutions of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] and use them to make solutions of HAuCl<sub>4</sub> and BSA at varying mole ratios.
 
*'''[[User:Abigail E. Miller|Abigail E. Miller]] 14:02, 17 September 2012 (EDT)''':didn't you also make various ratio solutions foHAuCl4 to BSA to synthesize AuNPs?


==Procedure==
==Procedure==
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## 3.75*10^-7mol [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] * 66,463g/mol [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] = .0249g [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] needed
## 3.75*10^-7mol [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] * 66,463g/mol [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] = .0249g [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] needed
## .0249g [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] was put in 25mL to make a 15μM solution
## .0249g [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] was put in 25mL to make a 15μM solution
#Solutions were made with the mole ratios of ([[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]]/[[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]]) 60, 80, 100, 120, 128, 130, 132, 133, 134, 136, 138, 140, 160, and 170. Volume of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution was calculated for each tube and inserted. The water needed for each tube was calculated by subtracting the volume of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] stock solution from 6mL.
##m<sub>1</sub>v<sub>1</sub>=m<sub>2</sub>v<sub>2</sub>
##15μM*(volume [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] stock solution) = (1.5*6)
###0.6mL [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] stock solution in each tube
##7720μM*x mL=6mL*(1.5*60μM)
###0.069mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
###5.331 mL H<sub>2</sub>O
##7720μM*x mL=6mL*(1.5*80μM)
###0.093mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
###5.307mL H<sub>2</sub>O
##7720μM*x mL=6mL*(1.5*100μM)
###0.117mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
###5.283mL H<sub>2</sub>O
##7720μM*x mL=6mL*(1.5*120μM)
###0.134mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
###5.266mL H<sub>2</sub>O
##7720μM*x mL=6mL*(1.5*128μM)
###0.149mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
###5.251mL H<sub>2</sub>O
##7720μM*x mL=6mL*(1.5*130μM)
###.152mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
###5.248mL H<sub>2</sub>O
##7720μM*x mL=6mL*(1.5*132μM)
###0.154mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
###5.246mL H<sub>2</sub>O
##7720μM*x mL=6mL*(1.5*133μM)
###0.155mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
###5.245mL H<sub>2</sub>O
##7720μM*x mL=6mL*(1.5*134μM)
###0.156mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
###5.254mL H<sub>2</sub>O
##7720μM*x mL=6mL*(1.5*136μM)
###0.159mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
###5.244mL H<sub>2</sub>O
##7720μM*x mL=6mL*(1.5*138μM)
###0.161mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
###5.242mL H<sub>2</sub>O
##7720μM*x mL=6mL*(1.5*140μM)
###0.163mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
###5.237mL H<sub>2</sub>O
##7720μM*x mL=6mL*(1.5*160μM)
###0.187mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
###5.213mL H<sub>2</sub>O
##7720μM*x mL=6mL*(1.5*170μM)
###0.198mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
###5.202mL H<sub>2</sub>O
##The tubes were wrapped in tin foil and heated at 80°C for 4 hours.


==Results==
==Results==

Revision as of 11:19, 18 September 2012

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Objective

  • Make stock solutions of HAuCl4 and BSA and use them to make solutions of HAuCl4 and BSA at varying mole ratios.

Procedure

  1. A stock solution of HAuCl4 was made, with the attempted Molarity of 10mM.
    1. (moles HAuCl4)/.025L H2O = .01M
    2. 2.5*10-4 mol HAuCl4 is the amount of gold needed to make this concentration with .025L
    3. 2.5*10-4 mol * 339.785mol/g HAuCl4 = .0849g HAuCl4 is needed
    4. .0197g of the .0849g stuck to the weigh paper, leaving .0656gHAuCl4.
    5. .0656g HAuCl4 * (1molHAuCl4/339.79g/molHAuCl4)= xM HAuCl4
    6. .007721M HAuCl4 was the molarity of stock solution made
    7. .0849g HAuCl4 was put in 25mL to make a 10mM solution
  2. A 15μM stock solution of BSA was made
    1. (moles BSA)/.025L = .000015
    2. 3.75*10^-7mol BSA is the amount of BSA needded to make a 15μM solution with .025L.
    3. 3.75*10^-7mol BSA * 66,463g/mol BSA = .0249g BSA needed
    4. .0249g BSA was put in 25mL to make a 15μM solution
  3. Solutions were made with the mole ratios of (HAuCl4/BSA) 60, 80, 100, 120, 128, 130, 132, 133, 134, 136, 138, 140, 160, and 170. Volume of HAuCl4 stock solution was calculated for each tube and inserted. The water needed for each tube was calculated by subtracting the volume of HAuCl4 stock solution and BSA stock solution from 6mL.
    1. m1v1=m2v2
    2. 15μM*(volume BSA stock solution) = (1.5*6)
      1. 0.6mL BSA stock solution in each tube
    3. 7720μM*x mL=6mL*(1.5*60μM)
      1. 0.069mL HAuCl4 stock solution
      2. 5.331 mL H2O
    4. 7720μM*x mL=6mL*(1.5*80μM)
      1. 0.093mL HAuCl4 stock solution
      2. 5.307mL H2O
    5. 7720μM*x mL=6mL*(1.5*100μM)
      1. 0.117mL HAuCl4 stock solution
      2. 5.283mL H2O
    6. 7720μM*x mL=6mL*(1.5*120μM)
      1. 0.134mL HAuCl4 stock solution
      2. 5.266mL H2O
    7. 7720μM*x mL=6mL*(1.5*128μM)
      1. 0.149mL HAuCl4 stock solution
      2. 5.251mL H2O
    8. 7720μM*x mL=6mL*(1.5*130μM)
      1. .152mL HAuCl4 stock solution
      2. 5.248mL H2O
    9. 7720μM*x mL=6mL*(1.5*132μM)
      1. 0.154mL HAuCl4 stock solution
      2. 5.246mL H2O
    10. 7720μM*x mL=6mL*(1.5*133μM)
      1. 0.155mL HAuCl4 stock solution
      2. 5.245mL H2O
    11. 7720μM*x mL=6mL*(1.5*134μM)
      1. 0.156mL HAuCl4 stock solution
      2. 5.254mL H2O
    12. 7720μM*x mL=6mL*(1.5*136μM)
      1. 0.159mL HAuCl4 stock solution
      2. 5.244mL H2O
    13. 7720μM*x mL=6mL*(1.5*138μM)
      1. 0.161mL HAuCl4 stock solution
      2. 5.242mL H2O
    14. 7720μM*x mL=6mL*(1.5*140μM)
      1. 0.163mL HAuCl4 stock solution
      2. 5.237mL H2O
    15. 7720μM*x mL=6mL*(1.5*160μM)
      1. 0.187mL HAuCl4 stock solution
      2. 5.213mL H2O
    16. 7720μM*x mL=6mL*(1.5*170μM)
      1. 0.198mL HAuCl4 stock solution
      2. 5.202mL H2O
    17. The tubes were wrapped in tin foil and heated at 80°C for 4 hours.

Results

  • The BSA solution created was 15μM
  • The actual molarity for the HAuCl4 stock solution created turned out to be 7.721mM rather than 10mM. This is because unlike BSA, which is easy to handle, HAuCl4 comes in large chunks which don't break easily and is hydroscopic, leaving residue on the weigh paper.


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