User:Michael F. Nagle/Notebook/Chem 571/2012/08/29: Difference between revisions
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==Procedure== | ==Procedure== | ||
#25mL stock solution of 10mM [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] was prepared. | #25mL of stock solution of 10mM [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] was prepared. | ||
## (moles [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]])/.025L H<sub>2</sub>O = .01M | ## (moles [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]])/.025L H<sub>2</sub>O = .01M | ||
## 2.5*10<sup>-4</sup> mol [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] is the amount of gold needed to make this concentration with .025L | ## 2.5*10<sup>-4</sup> mol [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] is the amount of gold needed to make this concentration with .025L | ||
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## .007721M [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] was the molarity of stock solution made | ## .007721M [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] was the molarity of stock solution made | ||
## .0849g [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] was put in 25mL to make a 10mM solution | ## .0849g [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] was put in 25mL to make a 10mM solution | ||
# | #25mL stock solution of 15μM [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] was prepared | ||
## (moles [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]])/.025L = .000015 | ## (moles [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]])/.025L = .000015 | ||
## 3.75*10^-7mol [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] is the amount of BSA needded to make a 15μM solution with .025L. | ## 3.75*10^-7mol [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] is the amount of BSA needded to make a 15μM solution with .025L. | ||
## 3.75*10^-7mol [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] * 66,463g/mol [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] = .0249g [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] needed | ## 3.75*10^-7mol [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] * 66,463g/mol [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] = .0249g [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] needed | ||
## .0249g [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] was put in 25mL to make a 15μM solution | ## .0249g [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] was put in 25mL to make a 15μM solution | ||
# | #6mL solutions were prepared with the mole ratios of ([[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]]/[[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]]) 60, 80, 100, 120, 128, 130, 132, 133, 134, 136, 138, 140, 160, and 170. .6mL of HAuCl<sub>4</sub> stock was added to each tube, so each was 1.5mM HAuCl<sub>4</sub> | ||
##m<sub>1</sub>v<sub>1</sub>=m<sub>2</sub>v<sub>2</sub> | |||
##The formula m<sub>1</sub>v<sub>1</sub>=m<sub>2</sub>v<sub>2</sub> was used to determine how much BSA was needed for mole ratios (BSA/Au) of 60-170 | |||
##15μM*(volume [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] stock solution) = (1.5*6) | ##15μM*(volume [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] stock solution) = (1.5*6) | ||
###0.6mL [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] stock solution in each tube | ###0.6mL [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] stock solution in each tube | ||
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###0.198mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution | ###0.198mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution | ||
###5.202mL H<sub>2</sub>O | ###5.202mL H<sub>2</sub>O | ||
##The tubes were wrapped in tin foil and heated at | ##The tubes were wrapped in tin foil and heated at 85°C for 4 hours. | ||
==Results== | ==Results== |
Revision as of 18:51, 6 December 2012
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