User:Michael F. Nagle/Notebook/Chem 571/2012/08/29: Difference between revisions
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==Objective== | |||
*Prepare stock solutions of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] and use them to make solutions of HAuCl<sub>4</sub> and BSA at varying mole ratios. | |||
**These are to be analyzed by UV/Vis [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/04|next week]] to find the optimal mole ratio for nucleation of gold nanoparticles. | |||
==Procedure== | ==Procedure== | ||
# | #25mL of stock solution of 7.721mM [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] was prepared. | ||
## (moles [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]])/.025L H<sub>2</sub>O = .01M | ## (moles [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]])/.025L H<sub>2</sub>O = .01M | ||
## 2.5*10<sup>-4</sup> mol [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] | ## 2.5*10<sup>-4</sup> mol [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] is the amount of gold needed to make this concentration with .025L | ||
## 2.5*10<sup>-4</sup> mol * 339.785mol/g [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] = .0849g | ## 2.5*10<sup>-4</sup> mol * 339.785mol/g [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] = .0849g HAuCl<sub>4</sub> is needed | ||
## .0197g of the .0849g stuck to the weigh paper, leaving .0656g[[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]]. | ## .0197g of the .0849g stuck to the weigh paper, leaving .0656g[[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]]. | ||
## .0656g [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] * (1mol[[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]]/339.79g/mol[[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]])= xM [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] | ## .0656g [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] * (1mol[[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]]/339.79g/mol[[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]])= xM [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] | ||
## .007721M [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] | ## .007721M [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] was the molarity of stock solution made | ||
## .0849g [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] was put in 25mL to make a 10mM solution | ## .0849g [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] was put in 25mL to make a 10mM solution | ||
# | #25mL stock solution of 15μM [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] was prepared | ||
## (moles [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]])/.025L = .000015 | ## (moles [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]])/.025L = .000015 | ||
## 3.75*10^-7mol [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] | ## 3.75*10^-7mol [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] is the amount of BSA needded to make a 15μM solution with .025L. | ||
## 3.75*10^-7mol [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] * 66,463g/mol [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] = .0249g [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] | ## 3.75*10^-7mol [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] * 66,463g/mol [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] = .0249g [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] needed | ||
## .0249g [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] was put in 25mL to make a 15μM solution | ## .0249g [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] was put in 25mL to make a 15μM solution | ||
# | #6mL solutions were prepared with the mole ratios of ([[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]]/[[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]]) 60, 80, 100, 120, 128, 130, 132, 133, 134, 136, 138, 140, 160, and 170. .6mL of HAuCl<sub>4</sub> stock was added to each tube, so each was 1.5mM HAuCl<sub>4</sub> | ||
*The formula m<sub>1</sub>v<sub>1</sub>=m<sub>2</sub>v<sub>2</sub> was used to determine how much BSA was needed for mole ratios (BSA/Au) of 60-170 | |||
**15μM*(volume [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] stock solution) = (1.5*6) | |||
***0.6mL [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] stock solution in each tube | |||
**7720μM*x mL=6mL*(1.5*60μM) | |||
***0.069mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution | |||
***5.331 mL H<sub>2</sub>O | |||
**7720μM*x mL=6mL*(1.5*80μM) | |||
***0.093mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution | |||
***5.307mL H<sub>2</sub>O | |||
**7720μM*x mL=6mL*(1.5*100μM) | |||
***0.117mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution | |||
***5.283mL H<sub>2</sub>O | |||
**7720μM*x mL=6mL*(1.5*120μM) | |||
***0.134mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution | |||
***5.266mL H<sub>2</sub>O | |||
**7720μM*x mL=6mL*(1.5*128μM) | |||
***0.149mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution | |||
***5.251mL H<sub>2</sub>O | |||
**7720μM*x mL=6mL*(1.5*130μM) | |||
***.152mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution | |||
***5.248mL H<sub>2</sub>O | |||
**7720μM*x mL=6mL*(1.5*132μM) | |||
***0.154mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution | |||
***5.246mL H<sub>2</sub>O | |||
**7720μM*x mL=6mL*(1.5*133μM) | |||
***0.155mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution | |||
***5.245mL H<sub>2</sub>O | |||
**7720μM*x mL=6mL*(1.5*134μM) | |||
***0.156mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution | |||
***5.254mL H<sub>2</sub>O | |||
**7720μM*x mL=6mL*(1.5*136μM) | |||
***0.159mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution | |||
***5.244mL H<sub>2</sub>O | |||
**7720μM*x mL=6mL*(1.5*138μM) | |||
***0.161mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution | |||
***5.242mL H<sub>2</sub>O | |||
**7720μM*x mL=6mL*(1.5*140μM) | |||
***0.163mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution | |||
***5.237mL H<sub>2</sub>O | |||
**7720μM*x mL=6mL*(1.5*160μM) | |||
***0.187mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution | |||
***5.213mL H<sub>2</sub>O | |||
**7720μM*x mL=6mL*(1.5*170μM) | |||
***0.198mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution | |||
***5.202mL H<sub>2</sub>O | |||
**The tubes were capped with tin foil and heated at 85°C for 4 hours. | |||
__NOTOC__ | __NOTOC__ |
Revision as of 18:58, 6 December 2012
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Objective
Procedure
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