User:Michael F. Nagle/Notebook/Chem 571/2012/09/04: Difference between revisions

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==Data==
==Data==
[[Image:BSA_AuNP_Uv-Vis.xlsx]]
[[Image:BSA_AuNP_Uv-Vis.xlsx]]
*'''[[User:Abigail E. Miller|Abigail E. Miller]] 14:11, 17 September 2012 (EDT)''':please embed an image directly on the page.then you can reference directly to it
[[Image:Image:Aubsamajorpeak9412.JPG]]
 
==Discussion==
==Discussion==
* Concentrations of 128-134 should be retested because a significant drop in absorbancy was observed between these values and 120 and 138. Between the mole concentrations of 120 and 128, and 134-138, a significant change in peak height in seen. This indicates a difference in the amount of gold nanoparticles in solution. The range of 128-134 should be tested again because all other concentrations, higher and lower, have much higher peaks.  
* Concentrations of 128-134 should be retested because a significant drop in absorbancy was observed between these values and 120 and 138. Between the mole concentrations of 120 and 128, and 134-138, a significant change in peak height in seen. This indicates a difference in the amount of gold nanoparticles in solution. The range of 128-134 should be tested again because all other concentrations, higher and lower, have much higher peaks.  

Revision as of 11:53, 18 September 2012

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Objective

  • Using UV/Vis, find which mole ratios of HAuCl4 to BSA result in the most BSA unfolding and formation of formation of protein fibers that include gold nanoparticles.
  • Make Tris stock solutions and do serial dilution with HAuCl4 and BSA fibers in Tris buffer

Procedure

  1. UV/Vis
    1. On 8.29, samples were made with varying mole concentration of HAuCl4 and BSA. .1mL of each solution was inserted into a cuvette, which went into a UV/Vis Spectroscoper
    1. Cuvette was cleaned between each spectra, and the same cuvette was used each time
  2. Tris buffer serial dilution
    1. .1M solution of Tris was needed to begin serial dilutions.
      1. .1M Tris * .1L = .01mol
      2. .01mol Tris * 121.14g/1mol = 1.21g Tris
      3. Stock solution of Tris was made at pH 8 and 10.
        1. 1.21g Tris was added to each tube, with 85mL H2O
        2. HCl was added dropwise until the pH changed to 8 and 10 in each tube, as indicated by a pH meter. 9mL HCl was added for pH=8 and 2mL was used for ph=10.
        3. Sufficient water was added to leave the final volume of the solution at 100mL.
    2. 9mL H2O was put in the first tube to receive 1mL Tris from the stock solution.
    3. 1mL was taken from the tube with a pipette and moved to the next tube. 1mL from this tube was moved to the next tube, and so on until all get some amount of Tris.

Data

File:BSA AuNP Uv-Vis.xlsx File:Image:Aubsamajorpeak9412.JPG

Discussion

  • Concentrations of 128-134 should be retested because a significant drop in absorbancy was observed between these values and 120 and 138. Between the mole concentrations of 120 and 128, and 134-138, a significant change in peak height in seen. This indicates a difference in the amount of gold nanoparticles in solution. The range of 128-134 should be tested again because all other concentrations, higher and lower, have much higher peaks.
  • Abigail E. Miller 14:11, 17 September 2012 (EDT):what hypothesis are you testing? did you centrifuge the samples? should you expect a drop in AuNP concentration with fiber formation between 128 and 134? make a graph of Au/BSA ratio versus absorbacne at 520-540 nm max. wavelength.
  • Dissolution of fibers in Tris should be tested at pH's 8 and 10 because Tris's optimal range is 7-9. Previous experiments used a pH of 10, which should also be tested so these can be replicated.


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