User:Michael F. Nagle/Notebook/Chem 571/2012/09/04: Difference between revisions

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*UV/Vis
*UV/Vis
**.1mL of each Au/BSA solution was inserted into a quartz cuvette, which went into a Shimadzu UV-2550 Spectrophotometer.
**.1mL of each Au/BSA solution was inserted into a quartz cuvette, which went into a Shimadzu UV-2550 Spectrophotometer.
***These are not the same solutions prepared [[User:Michael_F._Nagle/Notebook/Chem_571/2012/08/29|8/29]], as the HAuCl<sub>4</sub> did not react, perhaps due to contamination from using a metal spatula that was not wrapped in parafilm. The solutions used here were prepared by [[User:Abigail_E._Miller|Dr. Miller]
***These are not the same solutions prepared [[User:Michael_F._Nagle/Notebook/Chem_571/2012/08/29|8/29]], as the HAuCl<sub>4</sub> did not react, perhaps due to contamination from using a metal spatula that was not wrapped in parafilm. The solutions used here were prepared by [[User:Abigail_E._Miller|Dr. Miller]]
**Cuvette was cleaned between each spectra and the same cuvette was used each time
**Cuvette was cleaned between each spectra and the same cuvette was used each time
*Tris buffer serial dilution
*Tris buffer serial dilution

Revision as of 19:30, 6 December 2012

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Objective

  • Using UV/Vis, find optimal mole ratio of HAuCl4 to BSA for nucleation of gold nanoparticles in solution.
  • Prepare .1M Tris buffer at pH's of 8 and 10 to be added, at a wide range of concentrations, to solutions with Au/BSA fibers

Procedure

  • UV/Vis
    • .1mL of each Au/BSA solution was inserted into a quartz cuvette, which went into a Shimadzu UV-2550 Spectrophotometer.
      • These are not the same solutions prepared 8/29, as the HAuCl4 did not react, perhaps due to contamination from using a metal spatula that was not wrapped in parafilm. The solutions used here were prepared by Dr. Miller
    • Cuvette was cleaned between each spectra and the same cuvette was used each time
  • Tris buffer serial dilution
    • After being analyzed via UV/Vis, all HAuCl4 and BSA solutions were centrifuged. The fibers became stuck along the side of the tubes and fluid was dumped.
    • .1M solution of Tris was prepared at pH's of 8 and 10 by Puja Mody
    • 9mL H2O was added to each tube. A serial dilution was completed using Tris buffer at pH of 8.
    • 1mL Tris stock was added to the first tube. 1mL from this tube was moved to the next tube, and so on for the 12 remaining tubes with fibers.

Data

File:BSA AuNP Uv-Vis.xlsx

Discussion

  • Concentrations of 128-134 should be retested because a significant drop in absorbancy was observed between these values and 120 and 138. Between the mole concentrations of 120 and 128, and 134-138, a significant change in peak height in seen. This indicates a difference in the amount of gold nanoparticles in solution. The range of 128-134 should be tested again because all other concentrations, higher and lower, have much higher peaks.
  • Dissolution of fibers in Tris should be tested at pH's 8 and 10 because Tris's optimal range is 7-9. Previous experiments used a pH of 10, which should also be tested so these can be replicated.


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