User:Michael F. Nagle/Notebook/Chem 571/2012/09/04: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 7: Line 7:
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Objective==
==Objective==
* Using UV/Vis, find which mole ratios of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] to [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] result in the most [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] unfolding and formation of formation of protein fibers that include gold nanoparticles.
* Using UV/Vis, find optimal mole ratio of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] to [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] for nucleation of gold nanoparticles in solution.
*Make Tris stock solutions and do serial dilution with [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] fibers in Tris buffer
* Resuspend Au/BSA fibers with Tris buffer
**Find optimal pH and concentration of Tris for resuspension


==Procedure==
==Procedure==

Revision as of 19:02, 6 December 2012

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objective

  • Using UV/Vis, find optimal mole ratio of HAuCl4 to BSA for nucleation of gold nanoparticles in solution.
  • Resuspend Au/BSA fibers with Tris buffer
    • Find optimal pH and concentration of Tris for resuspension

Procedure

  1. UV/Vis
    1. On 8.29, samples were made with varying mole concentration of HAuCl4 and BSA. .1mL of each solution was inserted into a cuvette, which went into a UV/Vis Spectroscoper
    1. Cuvette was cleaned between each spectra, and the same cuvette was used each time
  2. Tris buffer serial dilution
    1. Solutions with varying mole ratios of HAuCl4 and BSA were centrifuged. The fibers became stuck along the side of the tubes and liquid was dumped.
    2. .1M solution of Tris was needed to begin serial dilutions.
      1. .1M Tris * .1L = .01mol
      2. .01mol Tris * 121.14g/1mol = 1.21g Tris
      3. Stock solution of Tris was made at pH 8 and 10.
        1. 1.21g Tris was added to each tube, with 85mL H2O
        2. HCl was added dropwise until the pH changed to 8 and 10 in each tube, as indicated by a pH meter. 9mL HCl was added for pH=8 and 2mL was used for ph=10.
        3. Sufficient water was added to leave the final volume of the solution at 100mL.
    3. 9mL H2O was put in the first tube to receive 1mL Tris from the stock solution.
    4. 1mL was taken from the tube with a pipette and moved to the next tube. 1mL from this tube was moved to the next tube, and so on until all get some amount of Tris.

Data

File:BSA AuNP Uv-Vis.xlsx

Discussion

  • Concentrations of 128-134 should be retested because a significant drop in absorbancy was observed between these values and 120 and 138. Between the mole concentrations of 120 and 128, and 134-138, a significant change in peak height in seen. This indicates a difference in the amount of gold nanoparticles in solution. The range of 128-134 should be tested again because all other concentrations, higher and lower, have much higher peaks.
  • Dissolution of fibers in Tris should be tested at pH's 8 and 10 because Tris's optimal range is 7-9. Previous experiments used a pH of 10, which should also be tested so these can be replicated.


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Michael_F._Nagle/Notebook/Chem_571/2012/09/04&oldid=662655"