User:Michael F. Nagle/Notebook/Chem 571/2012/09/11: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
No edit summary
Line 17: Line 17:
### 24.266μM * (volume) = 2.4266μM * 6mL
### 24.266μM * (volume) = 2.4266μM * 6mL
### .6mL BSA solution
### .6mL BSA solution
##Make new solution with mole ratio (HAuCl<sub>4</sub> to BSA) of 170.
## 2150μM HAuCl4 solution was used
## 2150μM HAuCl4 solution was used
### 2150μM * (volume) = 6*(2.4266*170)
### 2150μM * (volume) = 6*(2.4266*170)

Revision as of 11:36, 11 September 2012

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objectives

  • Use UV/Vis to find what mole ratios best allow unfolding of BSA and formation of gold nanoparticles from HAuCl4.
  • Obtain unfolded BSA fibers by reacting HAuCl4 and BSA at a mole ratio that allows unfolding
  1. UV/Vis was run on HAuCl4 and BSA solution with mole ratios of 120, 128, 130, 132, 133 and 134.
    1. The same cuvette was used and cleaned between each spectra.
  2. Solution was made with a mole ratio of 170
    1. 2.4268M BSA solution was used
      1. m1v1=m2v2
      2. 24.266μM * (volume) = 2.4266μM * 6mL
      3. .6mL BSA solution
    2. 2150μM HAuCl4 solution was used
      1. 2150μM * (volume) = 6*(2.4266*170)
      2. 1.151mL HAuCl4




Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Michael_F._Nagle/Notebook/Chem_571/2012/09/11&oldid=625134"