User:Michael F. Nagle/Notebook/Chem 571/2012/09/11: Difference between revisions

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### .474mL HAuCl<sub><sub>4</sub></sub> solution
### .474mL HAuCl<sub><sub>4</sub></sub> solution
==Data==
==Data==
[[Image:Chem-_Redone_AuNP-BSA.xlsx|Au/BSA solutions with mole ratios from 120-134]]
[[Image:Chem-_Redone_AuNP-BSA.xlsx||Au/BSA solutions with mole ratios from 120-134]]
[[Image:Tris_buffer_AuNP_UV-Vis_pH_8.xlsx|Au/BSA solutions with Tris at pH=8]]
[[Image:Tris_buffer_AuNP_UV-Vis_pH_8.xlsx||Au/BSA solutions with Tris at pH=8]]






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Revision as of 21:26, 13 September 2012

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Objectives

  • With UV/Vis, measure absorbance of BSA and formation of gold nanoparticles from HAuCl4 samples with mole ratios ranging from 120-134.
  • Measure absorbancy of samples created 9/5/12 with Tris buffer at pH=8 and compare spectra to those obtained last week for the same samples
  • Prepare solutions of HAuCl4 and BSA at a mole ratio that results in unfolded fibers in solution

Procedure

  1. UV/Vis was run on HAuCl4 and BSA solution with mole ratios of 120, 128, 130, 132, 133 and 134.
  2. UV/Vis was run on HAuCl4, BSA and Tris solutions. Spectra were compared to those obtained last week.
    1. The same cuvette was used and cleaned between each spectra.
  3. Solution was made with a mole ratio of 170
    1. 2.4268M BSA solution was used
      1. m1v1=m2v2
      2. 24.266μM * (volume) = 1μM * 6mL
      3. .247mL BSA solution
    2. 2150μM HAuCl4 solution was used
      1. 2150μM * (volume) = 6mL *(1μM*170)
      2. .474mL HAuCl4 solution

Data

File:Chem- Redone AuNP-BSA.xlsx File:Tris buffer AuNP UV-Vis pH 8.xlsx




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