User:Michael F. Nagle/Notebook/Chem 571/2012/09/11: Difference between revisions

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* With UV/Vis, measure absorbance of [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] and formation of gold nanoparticles from [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] samples with mole ratios ranging from 120-134. The samples were prepared last Wednesday, when two round of UV/Vis were run. The third round should show if the Tris has changed the dissolution of fibers.
* With UV/Vis, measure absorbance of [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] and formation of gold nanoparticles from [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] samples with mole ratios ranging from 120-134. The samples were prepared last Wednesday, when two round of UV/Vis were run. The third round should show if the Tris has changed the dissolution of fibers.
* Measure absorbancy of samples created [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/05|9/5/12]] with Tris buffer at pH=8 and compare spectra to those obtained last week for the same samples
* Measure absorbancy of samples created [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/05|9/5/12]] with Tris buffer at pH=8 and compare spectra to those obtained last week for the same samples
*'''[[User:Abigail E. Miller|Abigail E. Miller]] 14:43, 17 September 2012 (EDT)''':good (like to notebook pages)
* Prepare solutions of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] at a mole ratio that results in unfolded fibers in solution
* Prepare solutions of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] at a mole ratio that results in unfolded fibers in solution


Revision as of 13:31, 24 September 2012

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Objectives

  • With UV/Vis, measure absorbance of BSA and formation of gold nanoparticles from HAuCl4 samples with mole ratios ranging from 120-134. The samples were prepared last Wednesday, when two round of UV/Vis were run. The third round should show if the Tris has changed the dissolution of fibers.
  • Measure absorbancy of samples created 9/5/12 with Tris buffer at pH=8 and compare spectra to those obtained last week for the same samples
  • Prepare solutions of HAuCl4 and BSA at a mole ratio that results in unfolded fibers in solution

Procedure

  1. UV/Vis was run on HAuCl4 and BSA solution with mole ratios of 120, 128, 130, 132, 133 and 134.
  2. UV/Vis was run on HAuCl4, BSA and Tris solutions. Spectra were compared to those obtained last week.
  • Abigail E. Miller 14:43, 17 September 2012 (EDT):cite the dates and link to the notebook pages
    1. The same cuvette was used and cleaned between each spectra.
  1. Solution was made with a mole ratio of 170
    1. 2.4268M BSA solution was used
      1. m1v1=m2v2
      2. 24.266μM * (volume) = 1μM * 6mL
      3. .247mL BSA solution
    2. 2150μM HAuCl4 solution was used
      1. 2150μM * (volume) = 6mL *(1μM*170)
      2. .474mL HAuCl4 solution
  • Abigail E. Miller 14:43, 17 September 2012 (EDT):did you only make one sample or multiple? what did you do with the sample?

Data

File:Chem- Redone AuNP-BSA.xlsx
File:Tris buffer AuNP UV-Vis pH 8.xlsx

  • Abigail E. Miller 14:43, 17 September 2012 (EDT):embed images so the graphs are visible on the page

Discussion

  • No significant change was observed in the spectra for Tris solutions, indicating that no concentration affected the dissolution of fibers
  • Abigail E. Miller 14:40, 17 September 2012 (EDT):meaning no dissolution of hte fibers by Tris. at what pH?
  • Absorbance peaks were an order of magnitude or two greater than the first set of HAuCl4 and BSA solutions
  • Abigail E. Miller 14:40, 17 September 2012 (EDT):what does that mean? can you cite an example numerically or graphically?
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