Objectives
- With UV/Vis, measure absorbance of BSA and formation of gold nanoparticles from HAuCl4 samples with mole ratios ranging from 120-134.
- Measure absorbancy of samples created 9/5/12 with Tris buffer at pH=8 and compare spectra to the one obtained last week for the same samples
- Prepare Au/BSA fibers for resuspension at pH 10
Procedure
- UV/Vis was run on HAuCl4 and BSA solution with mole ratios of 120, 128, 130, 132, 133 and 134.
- UV/Vis was run on HAuCl4, BSA and Tris solutions. Spectra were compared to those obtained last week.
- The same microcuvette was used and cleaned between each spectra.
- Solution was made with a mole ratio of 170 using the same stock solutions and procedure as the 170 [Au/BSA] solution prepared 8/29. 8 solutions were prepared and they went in the oven at 80oC for 4 hours.
Data
Discussion
- Resuspension with tris: There was no change at 528nm, the wavelength of interest for AuNPs, relative to the background. However, the background decreases on the third trial.
- Au/BSA at various ratios: This time, the peak in absorbance at 528nm is at 130 rather than 128. This is interesting because in [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/05|last set], the peak was at 128 and abs. dropped to near zero at 130. Also, in the last set, abs. was an order of magnitude greater than this time.
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